Editor's note: To follow is a review of ocular drug delivery systems for the posterior segment currently in development. Because of the sheer volume of technologies, we chose to narrow this review by eliminating discussion of those that have US Food and Drug Administration or European Union approval for at least 1 indication. Figure 1 and Table 1 provide supplementary information for this article.
Nonbiodegradable polymeric drug delivery systems
• I-vation. Sustained delivery of triamcinolone acetonide (TA) to the vitreous using I-vation (Surmodics Inc.) is in development for diabetic macular edema (DME). The I-vation intravitreal implant is a titanium helical coil coated with TA (925 μg) and the nonbiodegradable polymers poly(methyl methacrylate) and ethylene-vinyl acetate. It is predicted that this implant will have an in vivo sustained delivery of at least 2 years. A phase 1 clinical trial in patients with DME has been completed and 24-month interim results have been reported.1 At 24 months, 25 of 31 patients remained in the study; 11 patients (11 eyes) in the slowrelease group, 14 patients (14 eyes) in the fast-release group. The proportion of patients demonstrating improved visual acuity (greater than 0 ETDRS letter gain from baseline) was 64% in the slow-release group and 72% in the fast-release group; 28.6% of patients in the fast-release group gained greater than 15 letters. The mean macular thickness, measured by optical coherence tomography (OCT), decreased in the slow group from 529 μm at baseline to 328 μm, and in the fast-release group from 376 μm at baseline to 268 μm. No further clinical trial has commenced.2
• Renexus. Encapsulated cell technology provides extracellular delivery of ciliary neurotrophic factor (CNTF) with long-term and stable intraocular release at constant doses through a device (Renexus [formerly NT-501], Neurotech) implanted in the vitreous. It contains human cell line of retinal pigment epithelium (RPE), NTC-200, genetically modified to secrete recombinant human CNTF. Renexus consists of a sealed semipermeable hollow fiber membrane capsule surrounding a scaffold of 6 strands of polyethylene terephthalate yarn, which can be loaded with cells. The device is surgically implanted in the vitreous through a tiny scleral incision and is anchored by a single suture through a titanium loop at one end of the device. The semipermeable hollow fiber membrane allows the outward diffusion of CNTF and other cellular metabolites and the inward diffusion of nutrients necessary to support the cell survival in the vitreous cavity while protecting the contents from host cellular immunologic attack.
Data from a phase 1 clinical trial showed that Renexus was well tolerated in patients with late-stage retinitis pigmentosa (RP) for 6 months.3 A phase 2 study in patients with geographic atrophy (GA) associated with atrophic age-related macular degeneration (AMD) randomized patients in a 2:1:1 ratio to receive high- (20 ng/day) or low-dose (5 ng/day) Renexus, or to sham surgery, respectively. Among eyes with baseline BCVA of 20/63, the mean BCVA in the high dose group was 10.5 and 10.0 letters greater than the low dose/ sham group at 12 months (P = .03) and 18 months, respectively.4,5 Stabilized visual acuity was accompanied by corresponding structural changes that were dose dependent (P < .001). The growth rate of GA area was reduced in treated eyes compared with fellow eyes at 12 and 18 months. In addition, Renexus prevented secondary cone degeneration in patients with RP.6 At present, a phase 2 clinical trial for early-stage RP or Usher syndrome (type 2 or 3) and a phase 1 study for macular telangiectasia are under way.
• NT-503. Neurotech has been developing another device (NT-503) encapsulating VEGF receptor Fc-fusion protein (VEGFR-Fc)-releasing cells.7 This VEGFR-Fc is 20-fold more efficient in neutralizing VEGF compared with ranibizumab. NT-503 is confirmed to release VEGFR-Fc constantly up to 1 year in the rabbit vitreous. A phase 1 clinical trial of NT-503 for neovascular AMD is ongoing outside of the United States.8
• ODTx. The injectable, biocompatible, nonresorbable device (ODTx, On Demand Therapeutics) contains reservoirs designed for drug release in optimized doses for laser activation.9,10 The reservoirs are capable of storing small- or large-molecule drugs that can be released via a standard, noninvasive laser activation procedure. The multiple reservoir system allows ophthalmologists to control drug delivery by activating specific reservoirs, while unactivated reservoirs remain intact. Unfortunately, clinical trials of ODTx have not commenced.
Biodegradable polymeric drug delivery systems
• Brimonidine intravitreal implant. Brimonidine is an alpha-2 adrenergic agonist that can release various neurotrophins, including BDNF, CNTF,11 and b-FGF.12 These neurotrophins have potential to prevent apoptosis of photoreceptors and/or retinal pigment epithelium.13,14 A phase 2/3 clinical trial of a biodegradable, rod-shaped poly(lactide-co-glycolide) intravitreal implant containing brimonidine tartrate in patients with retinitis pigmentosa has been completed, and a phase 2 clinical study in atrophic AMD15 is now being conducted by Allergan.
• Thethadur. Thethadur (pSivida) is a honeycomblike nanostructured porous silicon (biosilicon), which is both biocompatible and biodegradable. Thethadur can load a variety of drugs including small chemical entities, peptides, proteins, and other molecules and can provide sustained drug release.16 Further details regarding Thethadur have not been disclosed by the company.
• DE-102. In Japan, a randomized, multicenter, controlled, phase 2/3 clinical trial for sub-Tenon injection of biodegradable microspheres with betamethasone (DE-102, Santen Pharmaceutical Co. Ltd.) designed to sustained release has been conducted for the treatment of DME17 and for branch retinal vein occlusion.18
• Ranibizumab-loaded microspheres. Despite the remarkable effectiveness of ranibizumab (Lucentis, Genentech) for treating neovascular AMD and other retinal diseases, patients and physicians have been hoping for an alternative to frequent intravitreal injections. SurModics Inc. and Genentech have been developing biodegradable microparticles that incorporate ranibizumab, which are currently in preclinical stage.19 It is hoped that ranibizumab-loaded microparticles can deliver the agent over a period of approximately 4 to 6 months.
Oil/lipid-based drug delivery systems
• IBI-20089. Icon Bioscience Inc. is developing IBI-20089 containing TA using the Verisome drug delivery platform technology. The Verisome is a translucent liquid. When IBI-20089 mixes with saline, the solution becomes a milky, slightly opaque color and forms a gel. According to Icon's patent,20 IBI-20089 might be a solution of TA in benzyl benzoate. IBI-20089 is designed to last up to 1 year with a single intravitreal injection. An open-label, phase 1 study for cystoid macular edema associated with branch retinal vein occlusion or central retinal vein occlusion has been completed, and the results have been published.21 Five patients received an intravitreal injection of 6.9 μg TA in 25 μL, and another 5 patients received 13.8 μg TA in 50 μL using a 30-gauge needle. For the 6.9 μg cohort, mean central subfield thickness decreased from 477 μm at baseline to 369 μm at day 1 (P < .06); 387 μm at day 30 (P = .18), and 251 μm (P = .46) at day 360. For the 13.8 μg cohort, mean central subfield OCT thickness decreased from 518 μm at baseline to 404 μm at day 1 (P = .134); 289 μm at day 30 (P = .003), 207 μm at day 180 (P = .004), and 278 μm (P = .009) at day 360. The reported side effects include intraocular pressure (IOP) elevation, which occurred in 3 eyes, 2 of which had neovascular glaucoma.
IBI-20089 adjunctively with 0.5 mg of ranibizumab is now being tested in a phase 2 clinical study for neovascular AMD.22 Lim et al23 have demonstrated the safety and efficacy of a combination therapy of IBI-20089 and ranibizumab in 8 patients (3 patients; treatment-naive, 5 patients previously received ranibizumab injections) with neovascular AMD. Patients received intravitreal IBI- 20089 followed by intravitreal ranibizumab. At 30 days, 2 of 8 patients required reinjection of ranibizumab. At 60 (n=7) and 90 days (n=6), no patients required ranibizumab reinjections. At 120 days 3 of 6 patients, and at 180 days, 1 of 5 patients required repeated injections of ranibizumab. The results show that IBI-20089 may decrease the frequency of as-needed ranibizumab treatment.
• Cortiject. Cortiject (NOVA63035, Novagali Pharma SA, acquired by Santen Pharmaceutical Co. Ltd.) is a preservative-free emulsion composed of oily carrier and phospholipid as surfactant, encapsulating a target tissueactivated dexamethasone prodrug.24 Released dexamathasone prodrug is de-esterified by a retina-specific esterase, and activated to become dexamethasone. A single intravitreal injection provides sustained release over 6 to 9 months. An open-label, phase 1 study for DME is currently under way.25 According to an interim report,26 mean foveal thickness decreased after an intravitreal injection and continued lower at the last available follow-up time. However, further results of this trial have not been reported since 2009.
• Aganirsen. Eye drops of aganirsen (GS-101, GeneSignal International SA), an antisense oligonucleotide inhibiting insulin receptor substrate-1 expression, have been evaluated in a phase 3 clinical trial for the treatment of corneal angiogenesis associated with corneal transplantation.27 Recently, Cloutier et al28 evaluated emulsion-based eye drops of aganirsen applied in a laserinduced choroidal neovascularization (CNV) monkey model. A single topical application of aganirsen as an emulsion led to its dose-dependent accumulation in the retina within 90 minutes in monkeys. The incidence of high-grade CNV lesions decreased from 20.5% in vehicletreated animals to 1.7% (P < .05) at an 86-µg dose. Importantly, all doses of aganirsen led to a sustained concentration of the compound in the monkey retina up to 8 hours, and the concentration achieved in the retina was within the range of the effective concentration of aganirsen shown to prevent VEGF-induced capillary tube formation in human endothelial cells.
• iTrack microcatheter. The iTrack microcatheter (iScience Interventional) was originally designed for canaloplasty, a new treatment for glaucoma.29,30 The iTrack microcatheter includes an optical fiber to allow transmission of light to the microcannula tip for surgical illumination and guidance. The iTrack microcatheter has been evaluated for suprachoroidal drug delivery in animals. The pharmacokinetic study of suprachoroidal delivery of TA in pigs has shown that TA remained in the ocular tissues for at least 120 days, and systemic exposure was very low.31 In contrast, a study to compare the pharmacokinetics of bevacizumab (Avastin, Genentech) between intravitreal and suprachoroidal injections in pigs32 reported that the profile of intravitreal injections of bevacizumab was more sustained than that of suprachoroidal injections at the same dosage level. Intravitreally injected bevacizumab distributed more to the inner retina, whereas suprachoroidally injected bevacizumab distributed primarily to the choroid, RPE, and photoreceptor outer segments.
Tetz et al33 have reported the safety of suprachoroidal delivery of a combination of 4.0 mg bevacizumab and 4.0 mg TA via iTrack 400 microcatheter in 21 eyes of 21 advanced neovascular AMD patients who had been unresponsive to at least 3 prior other therapies including laser photocoagulation, photodynamic therapy, intravitreal pegaptanib (Macugen, Eyetech), bevacizumab, and ranibizumab. No serious intraoperative or postoperative complications including suprachoroidal hemorrhages were observed. One eye had a transient IOP elevation at 3 months after administration, which was medically controlled, and 2 eyes (10.5%) had apparent progression of nuclear sclerotic cataracts. Rizzo et al34 evaluated the safety and efficacy of suprachoroidal delivery of a combination of 1.25 mg bevacizumab and 4.0 mg TA via iTrack 400 microcatheter in 6 eyes with chronic macular edema with severe subfoveal hard exudates secondary to retinal vasculopathies including DME and RVO. BCVA improved by 2 lines or greater in 4 eyes and remained stable in 2 eyes. At 1 to 2 months after administration, hard exudates were almost completely resolved in all eyes, and macular edema was significantly reduced. There were no surgical or postoperative complications during 12 months of follow-up.
Recently, the iTrack microcatheter was used for cell transplantation into the subretinal space. A phase 1/2 clinical trial of human umbilical tissue-derived cells (CNTO 2476, Janssen Biotech Inc.) was conducted for atrophic AMD.35 A suspension of CNTO 2476 was delivered into the subretinal space near the macular geographic atrophy through a sclerotomy and choroidotomy via iTrack 275 microcatheter.36 In addition, de Smet et al37 evaluated the safety of repeated cell transplantation into the subretinal space in pigs via iTrack 275 microcatheter. Cells were successfully injected at the same or adjacent sites on the initial and the second dosing occasion without significant surgical trauma or adverse consequences in pigs.
• Microneedles. Microneedles, with lengths of 800 to 1000 µm, have successfully injected micro- and nanoparticles into the suprachoroidal space without surgery in rabbit, pig and human ex vivo eyes.38 The technology has promise for reducing the frequency of injection for treating retinal disease, and reducing exposure of potentially toxic compounds such as corticosteroid to the lens and other anterior segment structures of the eye.
Edelhauser et al38 performed a pharmacokinetic study of suprachoroidal injection of 2 mg TA in 100 µL into rabbit eyes in vivo, compared with intravitreal injection of the same dose.39 At 1 week after each injection, about 80% of TA was remained in both groups. However, in the intravitreal injection group, 63% was in the vitreous and more than 10% in the lens and anterior segment, whereas in the suprachoroidal injection group 77% remained in the choroid and negligible amounts reached the lens and anterior segment. At 2 months after each injection, approximately 30% of TA remained in the suprachoroidal injection group vs about 5% in the intravitreal injection group. In suprachoroidal injection of bevacizumab (1.25 mg/50 µL) using a microneedle 700-800 µm long, quick distribution of bevacizumab into the choroid was achieved (15 minutes; 76%), but choroidal levels declined very rapidly (2 days; 0.5%).40
Clearside Biomedical Inc. has been developing the lead product (CLS1001) for the treatment of macular edema.41
• MicroPump. A microelectromechanical systems (MEMS) drug delivery device is being investigated for the treatment of chronic and refractory ocular diseases.42,43 The MEMS device can be refilled with drug solution to provide long-term drug therapy while avoiding repeated surgeries. The first generation of MEMS is a manually controlled system limited by variations in the drug-release duration and force applied for depressing of the reservoir. To resolve this problem, the next generation device consists of an electrolysis chamber with electrolysis actuation to precisely delivery the desired dosage volume, a drug reservoir with refill port, battery and electronics. Biocompatible and flexible parylene is used to construct the MEMS. Battery and wireless inductive power transfer can be used to drive electrolysis. Electrolysis is a low power process in which the electrochemically induced phase change of water to hydrogen and oxygen gas generates pressure in the reservoir forcing the drug through the cannula.43 The reservoir is implanted in the subconjunctival space, and the flexible cannula is inserted through an incision into the anterior or posterior segment. Gonzalez-Soto et al44 have demonstrated that a slower prolonged infusion of the same volume and concentration of intravitreal ranibizumab is equivalent to a bolus intravitreal injection of ranibizumab in a human VEGF-induced retinal hyperpermeability model in rabbits.
Replenish Inc. plans to begin clinical trials for a refillable and programmable pump that would be implanted in the eye to deliver medicine for glaucoma or AMD. The Replenish device can last more than 5 years before needing replacement, much longer than current treatments.45
• Port drug delivery system. Genentech is also in collaboration with ForSight Vision 4 Inc., which is the fourth company to spin out of the ophthalmic incubator ForSight Labs, LLC, to develop a refillable port drug delivery system (PDS) designed to release ranibizumab over a period of months.46 Phase 1 studies of PDS have been conducted for the treatment of neovascular AMD by the company.47
Most chorioretinal and vitreoretinal diseases are chronic, progressive and refractory retinal degenerative diseases, and induced by complex pathophysiological conditions. It is necessary to consider further the most efficacious combinations of optimal drugs, doses, routes, and drug-release patterns (sustained-release, pulsatilerelease, or controlled-release responding to a trigger) based on the pathophysiology and progressive courses of the targeted diseases.
Noriyuki Kuno, PhD; and Shinobu Fujii are with the Research and Development Center at Santen Pharmaceutical Co. Ltd., in Nara, Japan. Dr. Kuno may be reached at +81 743 79 4508; fax: +81 743 79 4518; or via email at email@example.com.
- Dugel PU, Eliott D, Cantrill HL, Mahmoud T, Avery R, Erickson SR. I-VationTM TA: 24-month clinical results of the Phase I safety and preliminary efficacy study. Presented at the Association for Research in Vision and Ophthalmology Annual Meeting; May 2009; Fort Lauderdale, FL.
- ClinicalTrials.gov. A study of MK0140 in diabetic patients with macular edema. Available at: http://clinicaltrials. gov/ct2/show/NCT00692614?term=I-vation&rank=1.
- Sieving PA, Caruso RC, Tao W, et al. Ciliary neurotrophic factor (CNTF) for human retinal degeneration: phase I trial of CNTF delivered by encapsulated cell intraocular implants. Proc Natl Acad Sci U S A. 2006;103:3896-3901.
- Jaffe GJ, Tao W, Group CS. A Phase 2 study of encapsulated CNTF-secreting cell implant (NT-501) in patients with geographic atrophy associated with dry AMD-18 month results. Presented at the Association for Research in Vision and Ophthalmology Annual Meeting; May 2005; Fort Lauderdale, FL.
- Zhang K, Hopkins JJ, Heier JS, et al. Ciliary neurotrophic factor delivered by encapsulated cell intraocular implants for treatment of geographic atrophy in age-related macular degeneration. Proc Natl Acad Sci U S A. 2011;108:6241- 6245.
- Talcott KE, Ratnam K, Sundquist SM, et al. Longitudinal study of cone photoreceptors during retinal degeneration and in response to ciliary neurotrophic factor treatment. Invest Ophthalmol Vis Sci 2011;52:2219-2226.
- Kauper K, Elliot S, McGovern C, et al. Long-term, sustained intraocular delivery of escalating doses of VEGF antagonist using encapsulated cell technology implant for the treatment of choroidal neovascular diseases. Presented at the Association for Research in Vision and Ophthalmology Annual Meeting; May 2012; Fort Lauderdaule, FL.
- Ling V. Encapsulated cell technology: a new therapy for biologics delivery. Presented at the Annual Meeting of the Antibody Society; December 2011; San Diego.
- Laser-activated, on-demand drug delivery technology tested in vivo. Retina Today. Available at: http://bmctoday. net/retinatoday/2010/06/article.asp?f=laser-activated-on-demand-drug-delivery-technology-tested-in-vivo.
- The ODTx solution. Available at: http://www.ondemandtx.com/the-odtx-solution.aspx.
- Lonngren U, Napankangas U, Lafuente M, et al. The growth factor response in ischemic rat retina and superior colliculus after brimonidine pre-treatment. Brain Res Bull. 2006;71:208-218.
- Kim HS, Chang YI, Kim JH, Park CK. Alteration of retinal intrinsic survival signal and effect of alpha2-adrenergic receptor agonist in the retina of the chronic ocular hypertension rat. Vis Neurosci. 2007;24:127-139.33.
- Azadi S, Johnson LE, Paquet-Durand F, et al. CNTF+BDNF treatment and neuroprotective pathways in the rd1 mouse retina. Brain Res. 2007;1129:116-129.
- Zhang M, Mo X, Fang Y, et al. Rescue of photoreceptors by BDNF gene transfer using in vivo electroporation in the RCS rat of retinitis pigmentosa. Curr Eye Res. 2009;34:791-799.
- Safety and efficacy of brimonidine intravitreal implant in patients with geographic atrophy due to age-related macular degeneration (AMD). Available at: http://clinicaltrials.gov/ct2/show/NCT00658619.
- Products/Thethadur. Available at: http://psivida.com/products-biosilicon.html.
- Efficacy and safety of betamethasone micropsheres in patients with diabetic macular edema (Tsubasa). Available at: http://clinicaltrials.gov/ct2/show/NCT01411254?term=DE-102&rank=1.
- Efficacy and safety of betamethasone microspheres in patients with macular edema following branch retinal vein occlusion (HIKARI). Available at: http://clinicaltrials.gov/ct2/show/NCT01512901?term=DE-102&rank=2.
- SurModics enters ophthalmic lisence and development agreement with Roche and Genentech includes development and commercialization of a sustained drug delivery formulation of Lucentis and potential other Genentech compounds. Available at: http://phx.corporate-ir.net/phoenix.zhtml?c=80353&p=irol-newsArticle&ID=133900 1&highlight=genentech.
- Wong, V.; Wood, L. Conveniently Implantable Sustained Release Drug Compositions. US Patent WO2006/039336, 6 April 2006.
- Lim JI, Fung AE, Wieland M, Hung D, Wong V. Sustained-release intravitreal liquid drug delivery using triamcinolone acetonide for cystoid macular edema in retinal vein occlusion. Ophthalmology. 2011;118:1416-1422.
- IBI 20089+Lucentis combo intravitreal injections for treatment of neovascular age-related macular degeneration (AMD) (Icon Combo). http://clinicaltrials.gov/ct2/show/NCT01175395?term=IBI20089&rank=1. Accessed May 14, 2012.
- Lim JI, Niec M, Hung D, Wong V. A pilot study of combination therapy for neovascular AMD using a single injection of liquid sustained release intravitreal triamcinolone acetonide and intravitreal ranibizumab as needed. Presented at the Association for Research in Vision and Ophthalmology Annual Meeting; May 2012; Fort Lauderdale, FL.
- Eyeject. Available at: http://www.novagali.com/en/our-research/eyeject/
- Safety and tolerability of NOVA63035 “Corticosteroid” in patients with diabetic macular edema secondary to diabetic retinopathy. Available at: http://clinicaltrials.gov/ct2/show/NCT00665106?term=NOVA63035&rank=1.
- Kuppermann B. Cortiject dexamethasone lipid emulsion for the treatment of diabetic macular edema: A pilot study. The 8th International Symposium on Ocular Pharmacology and Therapeutics. 2009;33-31.
- GeneSignal. GeneSignal's aganirsen (GS-101) being developed in Phase III to treat corneal neovascularization. Available at: http://www.genesignal.com/fileadmin/user_upload/documents/Gene_Signal_Ophthal_HTA_ Meta_Analysis_Release_01-07-10.pdf.
- Cloutier F, Lawrence M, Goody R, et al. Antiangiogenic activity of aganirsen in nonhuman primate and rodent models of retinal neovascular disease after topical administration. Invest Ophthalmol Vis Sci. 2012;53:1195-1203.
- Lewis RA, von Wolff K, Tetz M, et al. Canaloplasty: circumferential viscodilation and tensioning of Schlemm canal using a flexible microcatheter for the treatment of open-angle glaucoma in adults. Two-year interim clinical study results. J Cataract Refract Surg. 2009;35:814-824.
- Kang SJ, Patel SR, Berezovsky DE, Zhang Q, Yang H, Grossniklaus HE. Suprachoroidal injection of microspheres with microcatheter in a rabbit model of uveal melanoma. Presented at the Association for Research in Vision and Ophthalmology Annual Meeting; May 2011; Fort Lauderdale, FL.
- Olsen TW, Feng X, Wabner K, et al. Cannulation of the suprachoroidal space: A novel drug delivery methodology to the posterior segment. Am J Ophthalmol. 2006;142:777-787.
- Olsen TW, Feng X, Wabner K, Csaky K, Pambuccian S, Cameron JD. Pharmacokinetics of pars plana intravitreal injections versus microcannula suprachoroidal injections of bevacizumab in a porcine model. Invest Ophthalmol Vis Sci. 2011;52:4749-4756.
- Tetz M, Rizzo S, Augustin AJ. Safety of submacular suprachoroidal drug administration via a microcatheter: retrospective analysis of European treatment results. Ophthalmologica. 2012;227:183-189.
- Rizzo S, Ebert FG, Bartolo ED, et al. Suprachoroidal drug infusion for the treatment of severe subfoveal hard exudates. Retina. 2012;32:776-784.
- A study of the safety and efficacy of CNTO2476 in patients with age-related macular degeration. Available at: http://clinicaltrials.gov/ct2/show/NCT01226628?term=CNTO2476&rank=1.
- Ho AC. Human adult umbilical stem cells: Potential treatment for atrophic AMD. Retina Today. 2011;7:59-61.
- de Smet MD, Wyse S, Vezina M, Conston SR, Sachs C, Popma SH. Repeated ab-externo catheterization of the sub-retinal space using a microcatheter for targeted delivery of a cell therapy product in a pig model. Presented at the Association for Research in Vision and Ophthalmology Annual Meeting; May 2012; Fort Lauderdale, FL.
- Patel SR, Lin AS, Edelhauser HF, Prausnitz MR. Suprachoroidal drug delivery to the back of the eye using hollow microneedles. Pharm Res. 2011;28:166-176.
- Larkin H. Microneedles: suprachoroidal injections may allow long-lasting treatment for retinal disease. Eurotimes. 2011;16:28.
- Mansoor S, Patel SR, Tas C, et al. Pharmacokinetics and biodistribution of bevacizumab following suprachoroidal injection into the rabbit eye using a microneedle. Presented at the Association for Research in Vision and Ophthalmology Annual Meeting; 2012; Fort Lauderdale, FL.
- Clearside Biomedical. Clearside Biomedical launched with pipeline and technology focused on drugs delivered to the back of the eye. Available at: http://www.clearsidebio.com/images/news release clearside series a financing led by hatteras final.pdf.
- Lo R, Li PY, Saati S, Agrawal RN, Humayun MS, Meng E. A passive MEMS drug delivery pump for treatment of ocular diseases. Biomed Microdevices. 2009;959-970.
- Saati S, Lo R, Li PY, Meng E, Varma R, Humayun MS. Mini drug pump for ophthalmic use. Curr Eye Res. 2010;35:192-201.
- Gonzalez-Soto R, Brant-Fernandes RA, Humayun MS, Varma R, Journey M, Caffey S. Intravitreal infusion of ranibizumab with an infusion pump in rabbits. Presented at the Association for Research in Vision and Ophthalmology Annual Meeting; May 2011; Fort Lauderdale, FL.
- Flanigan J. Biotech tries to shrug off setbacks. The New York Times; 2009.
- Genentech. Genentech announces first milestone payment to device-maker ForSight VISION4, Inc. in development of sustained delivery Lucentis. Available at: http://www.gene.com/gene/news/press-releases/display. do?method=detail&id=13808.
- ClinicalTrials.gov. Safety and preliminary efficacy study of PDS-1.0 in patients with neovascular AMD. Available at: http://clinicaltrials.gov/ct2/show/NCT01177644?term=forsight+PDS&rank=1.